兔抗CHEK1(Phospho-Ser280)多克隆抗体

Background:

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC2, CDC25B and CDC25C. Phosphorylation of CDC2 at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC2 and CDC25C. Phosphorylation of CDC2 at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC2. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Applications:

WB

Name of antibody:

CHEK1 (Phospho-Ser280)

Immunogen:

Synthetic peptide of human CHEK1 (Phospho-Ser280)

Full name:

checkpoint kinase 1 (Phospho-Ser280)

Synonyms：

CHK1

SwissProt:

O14757

WB Predicted band size:

54 kDa

WB Positive control:

HepG2 cells untreated or treated with UV

WB Recommended dilution:

500-1000