重组TIM-3蛋白人/鼠/猴/狗多种属

TIM-3蛋白产品信息

分子别名（Synonym）
HAVCR2,TIM3,TIMD3,FLJ14428,KIM3

表达区间及表达系统（Source）
Human TIM-3, His Tag (TM3-H5229) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1).
Predicted N-terminus: Ser 22

蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.8 kDa. The protein migrates as 44-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.

纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

质量管理体系（ISO, GMP）
质量优势
质控流程

分子背景（Background）
甲型肝炎病毒细胞受体2也称为HAVCR2、FLJ14428、KIM3、TIM3、TIMD3，是调节分子TIM家族的成员，具有一个Ig样V型结构域和富含Ser/Thr的粘蛋白柄。CD4阳性辅助T淋巴细胞根据其细胞因子分泌模式可分为1型（Th1）和2型（Th2）。Th1细胞及其相关细胞因子参与细胞介导的对细胞内病原体的和迟发型超敏反应，而Th2细胞参与控制细胞外蠕虫感染和促进特应性和过敏性疾病。这两种类型的细胞还交叉调节另一种细胞的功能。HAVCR2是一种Th1特异性细胞表面蛋白，调节巨噬细胞活化并增强小鼠实验性自身性脑脊髓炎的严重程度。HAVCR2调节巨噬细胞活化。抑制T辅助性1型淋巴细胞（Th1）介导的自身和同种反应，并促进耐受。也可能参与T细胞归巢。HAVCR2-半乳糖凝集素-9通路的失调可能是人类慢性自身性疾病状态的基础，如多发性硬化症。