兔抗Rasgrf1(Phospho-Ser916)多克隆抗体

Background:

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Applications:

IHC

Name of antibody:

Rasgrf1(Phospho-Ser916)

Immunogen:

Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.

Full name:

Ras protein-specific guanine nucleotide-releasing factor 1

Synonyms：

GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1

SwissProt:

Q13972

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100